Hyperbaric Oxygen Increases Stem Cell Proliferation, Angiogenesis and Wound-Healing Ability of WJ-MSCs in Diabetic Mice.

1. Front Physiol. 2018 Jul 30;9:995. doi: 10.3389/fphys.2018.00995. eCollection 2018.

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Peña-Villalobos I(1)(2), Casanova-Maldonado I(2), Lois P(2), Prieto C(2),
Pizarro C(2), Lattus J(3), Osorio G(4), Palma V(2).

Author information:
(1)Laboratorio de Ecofisiología Animal, Departamento de Ecología, Universidad de
Chile, Santiago, Chile.
(2)Laboratorio de Células Troncales y Biología del Desarrollo, Departamento de
Biología, Universidad de Chile, Santiago, Chile.
(3)Campus Oriente, Department of Obstetrics and Gynecology, Faculty of Medicine,
University of Chile, Santiago, Chile.
(4)Osorio Hermanos & Cia. Ltd., Quillota, Chile.

Hyperbaric oxygen therapy (HBOT) is effective for the medical treatment of
diverse diseases, infections, and tissue injury. In fact, in recent years there
is growing evidence on the beneficial effect of HBOT on non-healing ischemic
wounds. However, there is still yet discussion on how this treatment could
benefit from combination with regenerative medicine strategies. Here we analyzed
the effects of HBOT on three specific aspects of tissue growth, maintenance, and
regeneration: (i) modulation of adult rodent (Mus musculus) intestinal stem cell
turnover rates; (ii) angiogenesis dynamics during the development of the
chorio-allantoic membrane (CAM) in Gallus gallus embryos; (iii) and
wound-healing in a spontaneous type II diabetic mouse model with a low capacity
to regenerate skin. To analyze these aspects of tissue growth, maintenance, and
regeneration, we used HBOT alone or in combination with cellular therapy.
Specifically, Wharton Jelly Mesenchymal Stem cells (WJ-MSC) were embedded in a
commercial collagen-scaffold. HBOT did not affect the metabolic rate of adult
mice nor of chicken embryos. Notwithstanding, HBOT modified the proliferation
rate of stem cells in the mice small intestinal crypts, increased angiogenesis
in the CAM, and improved wound-healing and tissue repair in diabetic mice.
Moreover, our study demonstrates that combining stem cell therapy and HBOT has a
collaborative effect on wound-healing. In summary, our data underscore the
importance of oxygen tension as a regulator of stem cell biology and support the
potential use of oxygenation in clinical treatments.

DOI: 10.3389/fphys.2018.00995
PMCID: PMC6078002
PMID: 30104981